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BIOLOGICAL STUDIES ON THE BACTERIA OF THE MOUTH. 69
in the mouth of a patient suffering from acute gingivitis, a
spiriUum very similar to, if not identical with, the bacillus of
Finkler-Prior. Repeated efforts, made a few days later, to
obtain this bacterium from the same mouth, proved futile. In a
like manner I was able to isolate the vinegar bacterium from my
own saliva, after having drank a glass of beer in which this par-
ticular organism was present in large numbers; on the follow-
ing day my endeavors to find it failed, doubtless because this
bacterium cannot bear the high temperature of the mouth for
any length of time.
There are, however, a number of bacteria which almost
invariably occur in every mouth, and which may be termed
Mouth Bacteria Proper.
These are :
1. Leptothrix innoniinata.
2. Bacillus buccalis maximus.
3. Leptothrix buccalis maxima.
4. Jodococcus vaginatus.
5. Spirillum sputigenum.
6. Spirochtete dentium (dentieola).
These bacteria occur in every mouth ; sometimes even an
almost pure culture of Xo. 5 is found. They all have the pecul-
iarity that they will not grow on any of the usual culture media.
All endeavors at cultivation—and thousands have been made
proved unsuccessful. I have myself made hundreds of attempts
to cultivate them on all the various solid and fluid media. For
these experiments I had excellent material. My errand-boy had
a very carious right inferior molar, covered Avith tartar and
deposits, the surrounding gum being slightly inflamed. In the
cavity I found an almost pure culture of Spirillum sputigenum,
and on the margin of the gum one of Spirochsete dentium.
During several months I made cultures almost daily ; first, I
tried the usual Koch nutrient media, beef-water-peptone-gela-
tine, blood-serum, agar-agar, potato, etc. These experiments not
succeedins:, I varied the media bv addinti' difierent substances,
such as sugar, starch, etc., and by using saliva instead of water
as a solvent. I also employed weak alkaline and acid, as well as
BIOLOGICAL STUDIES ON THE BACTERIA OF THE MOUTH. 69
in the mouth of a patient suffering from acute gingivitis, a
spiriUum very similar to, if not identical with, the bacillus of
Finkler-Prior. Repeated efforts, made a few days later, to
obtain this bacterium from the same mouth, proved futile. In a
like manner I was able to isolate the vinegar bacterium from my
own saliva, after having drank a glass of beer in which this par-
ticular organism was present in large numbers; on the follow-
ing day my endeavors to find it failed, doubtless because this
bacterium cannot bear the high temperature of the mouth for
any length of time.
There are, however, a number of bacteria which almost
invariably occur in every mouth, and which may be termed
Mouth Bacteria Proper.
These are :
1. Leptothrix innoniinata.
2. Bacillus buccalis maximus.
3. Leptothrix buccalis maxima.
4. Jodococcus vaginatus.
5. Spirillum sputigenum.
6. Spirochtete dentium (dentieola).
These bacteria occur in every mouth ; sometimes even an
almost pure culture of Xo. 5 is found. They all have the pecul-
iarity that they will not grow on any of the usual culture media.
All endeavors at cultivation—and thousands have been made
proved unsuccessful. I have myself made hundreds of attempts
to cultivate them on all the various solid and fluid media. For
these experiments I had excellent material. My errand-boy had
a very carious right inferior molar, covered Avith tartar and
deposits, the surrounding gum being slightly inflamed. In the
cavity I found an almost pure culture of Spirillum sputigenum,
and on the margin of the gum one of Spirochsete dentium.
During several months I made cultures almost daily ; first, I
tried the usual Koch nutrient media, beef-water-peptone-gela-
tine, blood-serum, agar-agar, potato, etc. These experiments not
succeedins:, I varied the media bv addinti' difierent substances,
such as sugar, starch, etc., and by using saliva instead of water
as a solvent. I also employed weak alkaline and acid, as well as
