Page 93 - My FlipBook
P. 93
METHODS OF BACTEIilOLOGICAL INVESTIGATION. 67
Tissue Preparations.
It would lead us too far to attempt to give an idea of all the
many methods employed in staining bacteria in tissues, I refer
accordingly to only one or two :
Loffler^s method. The sections are brought for a few minutes
into the strong alkaline solution (concentrated alcoholic solu-
tion of methylene blue 30 c.cm., caustic potash (1 : 10,000)
100 c.cm.), then they arc washed in 0.5 per cent, acetic acid, de-
hydrated in absolute alcohol, cleared up in oil of cedar, and
mounted in Canada balsam.
Granfs method. Sections are placed first in alcohol, then in
the aniline w^ater-gentian violet solution a few minutes, then in
the iodine solution for one to three minutes, then in absolute
alcohol till they lose all visible color. Clear up in oil of cloves,
and mount in Canada balsam ; or, better, after Gunflur, stain one
minute, the iodine solution (page 51) two minutes, alcohol one-
half minute, 3 per cent, hydrochloric acid in alcohol exactly ten
seconds, then ak-ohol, oil of cloves, etc.
To obtain double coloring, bring the sections, stained in gen-
tian violet, from tbe alcohol into an aqueous solution of vesuvine
for some minutes, then again into absolute alcohol, dear up, and
mount in Canada balsam.
