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METHODS OF BACTERIOLOGICAL INVESTIGATION. 51

tripod; it is then removed, and the inner vessel filled with cold
(iced) water and the cover replaced. The outer vessel acts
simply as a receiver for the excess of water in the inner. A
small glass plate placed upon the cover is not only horizontal, but
is also cooled, so that in pouring the gelatine upon it there is no

Fio. 14

LeVELIM. Ari'AKAlLS.
For pouring culture-materials.
•danger of its running oft' at one side. For pouring agar-agar
the'simpler form of the apparatus represented in Fig. 14 should
be used.
12. A pair of scales (0.1-20.0 grams), a water-bath, one dozen
small glass dishes and watch-glasses, one dozen Petri'sche
Schalen (miniature damp chambers), measuring-glass, pipette,
test tubes, bottles of various size for holding preparations, also
some with ground stoppers for staining materials, a wash-bottle,
object- and cover-glasses, a few hollow object-glasses for drop-
cultures, various-sized porcelain evaporating-dishes, two platinum
needles, brushes, spatula, filters, filter-paper, cotton, wire baskets
for culture-tubes, scissors, pincers; further, for inoculation ex-
periments, a sterilizable syringe, two vaccination-needles, mice-
cages, etc.
The principal reagents made use of in staining bacteria are
absolute alcohol, aniline water,* cedar oil, oil of cloves. Gram's
solution (iodine 1, iodide of potassium 2, distilled water 300),
various staining materials, of which the most important are

*A saturated solution of aniline oil in water, made by thoroughly shaking
four parts aniline in one hundred parts water, then filtering.